SK-BR-3-CAS9 cell line

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SK-BR-3-CAS9 Order

Catalog#

YC-C048-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

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Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

SK-BR-3-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the SK-BR-3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a SK-BR-3-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
SK-BR-3-CAS9	Cas9	14.79796696	2.00070763
SK-BR-3-CAS9	β-actin	12.79725933	2.00070763
SK-BR-3	Cas9	33.42461395	20.89612579
SK-BR-3	β-actin	12.52848816	20.89612579

Cutting Efficiency Validation

SK-BR-3-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the SK-BR-3-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:SK-BR-3			STR Info (Cell bank) Cell Line:SK-BR-3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	9	12		9	12
D13S317	11	12		11	12
D7S820	9	9		9	12
D16S539	9	9		9	9
VWA	17	17		17	17
TH01	8	9		8	9
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	12	12		12	12
D12S391	18	20
FGA	20	20
D2S1338	20	25
D21S11	30	30.2
D18S51	10	13
D8S1179	12	12
D3S1358	17	17
D6S1043	11	19
PENTAE	10	11
D19S433	14	14
PENTAD	9	12
D1S1656	11	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

SK-BR-3-CAS9

Catalog#

YC-C048-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SK-BR-3-CAS9 cell line

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